S gene sequencing of SARS-CoV-2 clinical isolate - SRA

SRX14473548: S gene sequencing of SARS-CoV-2 clinical isolate
1 ILLUMINA (Illumina MiSeq) run: 55,463 spots, 15.8M bases, 7.9Mb downloads

Design: Viral RNA extraction on 1 mL of swab fluid by TRIzol-LS reagent, random primer cDNA synthesis by Invitrogen Superscript IV reverse transcriptase, S gene amplification using in-house nested PCR primers

Submitted by: Brigham and Women's Hospital

Study: SARS-CoV-2 Bamlanivimab resistance sequencing

PRJNA816433 • SRP364287 • All experiments • All runs

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As part of the ACTIV-2/A5401 randomized clinical trial (NCT04518410), non-hospitalized participants with symptomatic SARS-CoV-2 infection were given bamlanivimab (700mg or 7000mg) or placebo treatment. We performed targeted Spike (S) gene next-generation sequencing to detect bamlanivimab resistance mutations. Using this data, we can assess whether in vivo emergence of SARS-CoV-2 resistance mutations alters viral replication dynamics or therapeutic efficacy in the immune-competent population.

Sample: SARS-CoV-2 clinical targeted S gene sequencing

SAMN26675778 • SRS12274787 • All experiments • All runs

Organism: Severe acute respiratory syndrome coronavirus 2

Library:

Name: ACTIV2-1I835657295

Instrument: Illumina MiSeq

Strategy: AMPLICON

Source: VIRAL RNA

Selection: RT-PCR

Layout: SINGLE

Runs: 1 run, 55,463 spots, 15.8M bases, 7.9Mb

Run	# of Spots	# of Bases	Size	Published
SRR18337197	55,463	15.8M	7.9Mb	2022-03-18

ID:: 20654752

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